cell line jvm-3  (DSMZ)

Journal: NPJ genomic medicine

Article Title: PanCancer analysis of somatic mutations in repetitive regions reveals recurrent mutations in snRNA U2.

doi: 10.1038/s41525-022-00292-2

Figure Lengend Snippet: Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from CLL JVM3 cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.

Article Snippet: CLL cell line JVM3 (DSMZ) was grown in RPMI 1640, 10% FBS, 1% PSG and 1% non-essential amino acids.

Techniques: Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Control, Western Blot, Sequencing